production of monoclonal antibody against human nestin
نویسندگان
چکیده
we have employed a peptide-based antibody generation protocol for producing antibody against human nestin. using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any n- or o-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. a wide variety of nestin proteins ranging from 140-250 kda was detected by this antibody. this antibody is highly specific and functional in applications such as elisa, flow cytometry, immunocytochemistry, and western blot assays.
منابع مشابه
Production of Monoclonal Antibody against Human Nestin
We have employed a peptide-based antibody generation protocol for producing antibody against human nestin. Using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any N- or O-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. A wide variety of nestin proteins ranging from 140-250 kDa w...
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عنوان ژورنال:
avicenna journal of medical biotechnologyجلد ۲، شماره ۲، صفحات ۶۹-۷۷
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